Born in Lancaster, Massachusetts, Burbank grew up on a farm and received only an elementary school education. The thirteenth of eighteen children, he enjoyed the plants in his mother's large garden. His father died when he was 21 years old, and Burbank used his inheritance to buy a 17-acre (69,000 m²) plot of land near Lunenburg center. There, he developed the Burbank potato. Burbank sold the rights to the Burbank potato for $150 and used the money to travel to Santa Rosa, California, in 1875. Later, a natural sport of Burbank potato with russetted skin was selected and named Russet Burbank potato. Today, the Russet Burbank potato is the most widely cultivated potato in the United States. A large percentage of McDonald's french fries are made from this cultivar.
In Santa Rosa, Burbank purchased a 4-acre (16,000 m2) plot of land, and established a greenhouse, nursery, and experimental fields that he used to conduct crossbreeding experiments on plants, inspired by Charles Darwin's The Variation of Animals and Plants under Domestication. (This site is now open to the public as a city park, Luther Burbank Home and Gardens.) Later he purchased an 18-acre (7.3 ha) plot of land in the nearby town of Sebastopol, called Gold Ridge Farm.
From 1904 through 1909, Burbank received several grants from the Carnegie Institution to support his ongoing research on hybridization.
Burbank became known through his plant catalogs, the most famous being 1893's "New Creations in Fruits and Flowers," and through the word of mouth of satisfied customers, as well as press reports that kept him in the news throughout the first decade of the century.